immunohistochemical expression of pcna and ki-67 in periapical granuloma and radicular cyst

نویسندگان

s. sargolzaei dept of oral & maxillofacial pathology, school of dentistry, shahid beheshti university of medical sciences, tehran, iran.

a. roufegarinejad faculty of dentistry, mcgill university, montreal, canada

s. shamszadeh iranian center for endodontic research, research institute of dental science, shahid beheshti university of medical sciences, tehran, iran.

چکیده

objective: periapical granulomas (pgs) and radicular cysts (rcs), as the most common odontogenic lesions have yet unclear pathogenesis. this study was aimed to compare pcna and ki-67 expression in pgs and rcs and evaluate their possible relationship with two lesions. methods: in this cross-sectional descriptive study, twenty pgs and twenty rcs were evaluated immunohistochemically using an anti-pcna and anti-ki-67 polyclonal antibodies. pcna+ and ki-67+ cells were counted in connective tissue wall and epithelial lining (in rcs). statistical analysis was performed by using mann-whitney u test and spearman’s rank correlation coefficient results: in pgs, percentage of pcna and ki-67 expression were found 70% and 30%, respectively; in rcs, pcna and ki-67 expression were observed 90% and 55%, respectively. additionally, in rcs, immunoexpression of pcna (85%) and ki-67 (60%) were detected at epithelial lining area. the positive immunoexpression of pcna in rcs was greater than pgs (p<0.05). conclusion immunoexpression of pcna and ki-67 were detected in both lesions which may be mentioned as valuable markers for the prediction of biologic behavior of pgs and rcs.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Immunohistochemical Expression of PCNA and Ki-67 in Periapical Granuloma and Radicular Cyst

Objectives: Periapical Granulomas (PGs) and Radicular Cysts (RCs), as the most common odontogenic lesions have yet unclear pathogenesis. This study was aimed to compare PCNA and Ki-67 expression in PGs and RCs and evaluate their possible relationship with two lesions. Methods: In this cross-sectional descriptive study, twenty PGs and twenty RCs were evaluated immunohistochemically using an a...

متن کامل

A comparative study of PCNA and Ki-67 expression in dental follicle, dentigerous cyst, unicystic ameloblastoma and ameloblastoma

Various cell proliferation markers are used as diagnostic and prognostic tools in oral lesions. Simultaneous evaluation of these markers can increase the precision of estimation of the proliferative status of different tissues. In this study we investigated the expression of PCNA and Ki-67 as markers of cell proliferation in 15 paraffin embedded samples of each dental follicle, dentigerous cy...

متن کامل

Immunohistochemical Expression of Ki-67, PCNA and CD34 in Astrocytomas: A Clinicopathological Study.

OBJECTIVES To assess the immunohistochemical expression of Ki-67 and Proliferating Cell Nuclear Antigen (PCNA) as proliferative markers to study proliferative activity and CD34 as an endothelial cell marker in order to study vascular proliferation in astrocytomas in correlation with some clinicopathological parameters (age, gender, site of the tumor, and tumor grade). METHODS A retrospective ...

متن کامل

a comparative study of pcna and ki-67 expression in dental follicle, dentigerous cyst, unicystic ameloblastoma and ameloblastoma

various cell proliferation markers are used as diagnostic and prognostic tools in oral lesions. simultaneous evaluation of these markers can increase the precision of estimation of the proliferative status of different tissues. in this study we investigated the expression of pcna and ki-67 as markers of cell proliferation in 15 paraffin embedded samples of each dental follicle, dentigerous cyst...

متن کامل

Immunohistochemical detection of laminin-1 and Ki-67 in radicular cysts and keratocystic odontogenic tumors

BACKGROUND Odontogenic cysts are those which arise from the epithelium associated with the development of teeth. Some odontogenic cysts were found to have special biological features that make them distinct from other lesions. This study was conducted to detect the immunoepxression of laminin-1 and Ki-67 in both radicular cysts (RCs) and keratocystic odontogenic tumors (KCOTs) and to examine th...

متن کامل

Immunohistochemical Assessment of Ki-67 Expression in Adenoid Cystic Carcinoma of the Salivary Glands

Objective Adenoid cystic carcinoma (ACC) is a rare malignant tumor originating from the salivary glands. It’s rather bland histological appearance that masks its ultimate biological aggressiveness. Evaluation of cell cycle and mitoses have been useful in predicting malignancy in many tumors. Ki-67 antigen is a human nuclear antigen that presents during all active phases of cell cycle. The aim ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
journal of dental school, shahid beheshti university of medical sciences

جلد ۳۴، شماره ۱، صفحات ۵۸-۶۵

کلمات کلیدی
[ ' i m m u n o h i s t o c h e m i s t r y ' , ' k i ' , 6 7 , ' p c n a ' , ' p e r i a p i c a l g r a n u l o m a ' , ' r a d i c u l a r c y s t . ' ]

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023